cd44 apc  (Miltenyi Biotec)

Journal: Drug Design, Development and Therapy

Article Title: Hirsutella sinensis Fungus Enhances CD8 + T Cell Anti-Tumor Activity in NSCLC via Modulating the IL-10/STAT3/STAT1 Axis

doi: 10.2147/DDDT.S531106

Figure Lengend Snippet: HSF decreased TAMs and regulatory T cells and increased memory T cells population in the tumor microenvironment. ( A ) Percentage of TAMs (displayed by CD11b + F4/80 + staining) in tumor tissue. ( B ) Percentage of regulatory T cells (displayed by CD25 + CD127 − staining) in tumor tissue. ( C ) Percentage of CD4 + T cells (displayed by CD3 + CD4 + staining) in tumor tissue. ( D ) Percentage of CD8 + T cells (displayed by CD3 + CD8 + staining) in tumor tissue. Percentage of T(CM) (displayed by CD44 High CD62L High staining) and Naïve T cells (displayed by CD44 Low CD62L High staining) of ( E ) CD4 + and ( F ) CD8 + T cells in tumor tissue. Data were presented as each dot for one animal and the exact p shown on plot.

Article Snippet: Then, cells were placed on ice in FACS buffer for 30 min of staining with the following surface antibodies: F4/80 (1:500 dilution, F21480A02, MultiSciences, Hangzhou, China), CD11b (1:1000 dilution, F11011B03, MultiSciences), CD25 (1:200 dilution, F2102503, MultiSciences), CD127 (1:200 dilution, F2112702, MultiSciences), CD3 (1:200 dilution, AM003E0205-100, MultiSciences), CD4 (1:1000 dilution, SC-19641PERCP, Santa), CD8 (1:200 dilution, AM008A0204-100, MultiSciences), CD44 (1:200 dilution, F1104403, MultiSciences), and CD62L (1:500 dilution, 746726, BD Biosciences) for 1 h at room temperature.

Techniques: Staining

Journal: Drug Design, Development and Therapy

Article Title: Hirsutella sinensis Fungus Enhances CD8 + T Cell Anti-Tumor Activity in NSCLC via Modulating the IL-10/STAT3/STAT1 Axis

doi: 10.2147/DDDT.S531106

Figure Lengend Snippet: HSF failed to increase memory T cells population in IL-10 knockdown of LLC-bearing mice. ( A ) Percentage of TAMs (displayed by CD11b+F4/80+ staining) in xenograft tumors of IL-10 knockdown LLC bearing mice. ( B ) Percentage of CD4 + T cells (displayed by CD3 + CD4 + staining) in xenograft tumors of IL-10 knockdown LLC bearing mice. ( C ) Percentage of CD8 + T cells (displayed by CD3 + CD8 + staining) in xenograft tumors of IL-10 knockdown LLC bearing mice. ( D ) Percentage of regulatory T cells (displayed by CD25 + CD127 − staining) in xenograft tumors of IL-10 knockdown LLC bearing mice. Percentage of T(CM) (displayed by CD44 High CD62L High staining) and Naïve T cells (displayed by CD44 Low CD62L High staining) of ( E ) CD4 + and ( F ) CD8 + T cells in xenograft tumors of IL-10 knockdown LLC bearing mice. Data were displayed as each dot for one animal and the exact p shown on plot (n=8).

Article Snippet: Then, cells were placed on ice in FACS buffer for 30 min of staining with the following surface antibodies: F4/80 (1:500 dilution, F21480A02, MultiSciences, Hangzhou, China), CD11b (1:1000 dilution, F11011B03, MultiSciences), CD25 (1:200 dilution, F2102503, MultiSciences), CD127 (1:200 dilution, F2112702, MultiSciences), CD3 (1:200 dilution, AM003E0205-100, MultiSciences), CD4 (1:1000 dilution, SC-19641PERCP, Santa), CD8 (1:200 dilution, AM008A0204-100, MultiSciences), CD44 (1:200 dilution, F1104403, MultiSciences), and CD62L (1:500 dilution, 746726, BD Biosciences) for 1 h at room temperature.

Techniques: Knockdown, Staining

Journal: Breast Cancer Research : BCR

Article Title: IL-3/STAT5/miR-155-5p axis supports stem-related pathway reprogramming in TNBC

doi: 10.1186/s13058-025-02143-1

Figure Lengend Snippet: IL-3 boosts BCSC marker expression and mammosphere formation. ( A , B ) Representative FACS dot plots of ALDH1A1 and CD44/CD24 in MDA-MB-436 and ( C , D ) Hs-578T cells upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( E , F ) ALDH activity assay on supernatant of MDA-MB-436 and Hs-578T cell culture upon treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5 ng/ml) and NC (100 pmol) + IL-3 (5 ng/ml) for 24h (n = 3; 3 biological replicates for each n). Data are represented as mean ± SEM. ( G-H ) Mammosphere formation assay of MDA-MB-436 and Hs-578T cells subjected to treatment with saline, IL-3 (5 ng/ml), antagomiR-155 (100 pmol) + IL-3 (5ng/ml), NC (100 pmol) + IL-3 (5 ng/ml), IL-3Rα blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) and IL-3 blocking mAb (1 ng/ml) + IL-3 (5 ng/ml) (n = 3; 3 biological replicates for each n). Original magnification corresponds to 20X, scale bar: 50 µm. Data are represented as mean ± SEM

Article Snippet: The following anti-human antibodies were used: IL-3Rα-APC, (Milteny Biotec #130-113-322), CD44-FITC (Miteny Biotec #130-113-341), CD24-PE (Milteny Biotec #130-112-656), ALDH1A1-PE (abcam #ab209537).

Techniques: Marker, Expressing, Saline, Activity Assay, Cell Culture, Tube Formation Assay, Blocking Assay